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KMID : 0613820140240111224
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Journal of Life Science
2014 Volume.24 No. 11 p.1224 ~ p.1230
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Antiadipogenic Effects of Red Radish (Raphanus sativus L.) Sprout Extract in 3T3-L1 Preadipocytes
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Kim Da-Hye
Kim Sang-Jun
Jeong Seung-Il
Cheon Chun-Jin
Kim Seon-Young
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Abstract
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The red radish (Raphanus sativus L.; RR) sprout is a plant of the cruciferous family. In this study, we elucidated the effect of the water extract of RR sprout (RRSE) against α-amylase, α-glucosidase, and pancreatic lipase enzyme activity and adipogenesis in 3T3-L1 preadipocytes. α-amylase, α- glucosidase, and pancreatic lipase enzyme activity was inhibited in a concentration-dependent manner by RRSE treatment. RSSE also abolished adipocyte differentiation and lipid and triglyceride accumulation without cytotoxicity in 3T3-L1 adipocytes. In addition, RRSE modulated the expression of the proteins related to adipogenic transcription factors: peroxisome proliferator-activated receptor (PPAR)γ, sterol regulatory element-binding protein 1 (SREBP-1), and CCAT/enhancer binding protein (C/EBP)α. RRSE also suppressed expression of the proteins responsible for lipid synthesis, transport, and storage: adiponectin, fatty acid synthesis (FAS), perilipin, and fatty acid bind protein-4 (FABP4). This study showed that RRS treatment has the potential to inhibit obesity by controlling the expression of adipogenic transcription factors and adipogenic proteins.
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KEYWORD
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Adipogenesis, lipase activity, lipid accumulation, obesity, red radish sprout
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